Phage PVP-SE1 as Tool Recognition in Polydiacetylene to Produce Intelligent Packaging

Taila V. de Oliveira, Nilda de F. F. Soares, Carla M. Carvalho, Pablo Fuciños, Jane S. dos R. Coimbra, Nélio J. de Andrade, Eber A. A. Medeiros and Paulo P. Freitas
 

Abstract

Polydiacetylene (PDA) compound has unique chromatic properties when embedded in methylcellulose base. Therefore, this could be used as intelligent packaging. PDA exhibits blue-to-red color transition under certain stimuli, which was caused by alterations in PDA conjugation length backbone. For a specific color modification, bio-recognition tools were covalently linked with PDA. In this work, a broad-spectrum virulent phage (PVP-SE1) was used as a biorecognition element to detect Salmonella cells due to PDA color change. A series of experiments were performed to monitor colorimetric response. Consequently, chromatic immunoassay could be significant in increasing potential applications of PDA intelligent packaging.

Published on: May 31, 2017
doi: 10.17756/jfcn.2017-040
Citation:  de Oliveira TV, Soares N de FF, Carvalho CM, Fuciños P, dos R. Coimbra JS et al. 2017. Phage PVP-SE1 as Tool Recognition in Polydiacetylene to Produce Intelligent Packaging. J Food Chem Nanotechnol 3(2): 72-79.
 
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