Abstract
Rapid multi pesticide residue analysis methods are nowadays in demand to authentify food, agrochemical and environment samples. Aim of the study was to develop accurate and rapid method for analysis of dinotefuran, pyriproxyfen, and diafenthiuron by reverse phase ultrafast liquid chromatographic (RP-UFLC). The chromatographic conditions were optimized using a Shim-Pack GIST-HP (2.1 mm × 50 mm, 1.9 μ) column with a mobile phase comprising acetonitrile and water (65:35 v/v). Fine-tuning key parameters such as column’s temperature (40 °C), flow (0.6 mL per min) and wavelength (240 nm) enabled superior separation and sensitivity, ensuring minimal retention time and sharp peak resolution despite the complex matrix. Linearity studies demonstrated excellent correlation (R² > 0.999) over a wide concentration range, ensuring accurate quantification across varying concentrations. Intra- and inter-day precision values showed percent relative standard deviation (%RSD) well within 2.0%. The accuracy was validated through recovery rates ranging between 99.75% and 100.07% for all three active ingredients. Robustness tests revealed no significant variations in results under minor changes in chromatographic conditions. Furthermore, the method is suitable for residue samples including water, fruits, and vegetables ensuring comprehensive monitoring of presence in various matrices per SANCO guidelines.
doi: 10.17756/jfcn.2025-s2-005
Citation: Singh R, Sharma S, Dhalani J. 2025. Simultaneous Quantification of Dinotefuran, Pyriproxyfen, and Diafenthiuron by Reverse Phase Ultrafast Liquid Chromatography: Application to Food, Agrochemical, and Environment Samples. J Food Chem Nanotechnol 11(S2): S47-S53.
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