Isolation and Antimicrobial Activity of Plant-associated Lactic Acid Bacteria against Pantoea stewartii Subsp. stewartii of Jackfruit Bronzing Disease

Nur Sulastri Jaffar, Roslina Jawan and Khim Phin Chong

Abstract

Pantoea stewartii subsp. stewartia (Pss) is the causative agent of “Jackfuit Bronzing,” an emerging disease in jackfruit crops (Artocarpus heterophyllus L.). The disease was first discovered in Malaysia in 2017, affecting mostly the J33 clone, tekam yellow. This disease degrades the quality of fresh jackfruit, leading to economic losses, and has remained a serious problem for the Malaysian jackfruit trade. In this study, lactic acid bacteria (LAB) isolated from fruits and vegetables were screened and characterized for their antimicrobial potential against Pss. The LAB isolation process was carried out using the standard method, and the isolated colonies were initially distinguished by their morphological features on de Man, Rogosa and Sharpe (MRS) agar, Gram-positive staining characteristics, and catalase-negative responses. These colonies were then selected for identification through polymerase chain reaction (PCR) amplification of the 16S rDNA gene and sequencing. Fourteen cell-free supernatants (CFS) of the LAB isolates were found to significantly inhibit the growth of Pss in vitro (p < 0.05). LAB isolates showing promising potential as biocontrol agents were identified as Lactiplantibacillus plantarum, Leuconostoc holzapfelii, Weissella cibaria, and Weisella paramesenteroides. The findings of this study suggested that the isolated LAB may have antibacterial characteristics that could be investigated further for the treatment of jackfruit-bronzing disease.

Published on: November 15, 2023
doi: 10.17756/jfcn.2023-s1-079
Citation: Jaffar NS, Jawan R, Chong KP. 2023. Isolation and Antimicrobial Activity of Plant-associated Lactic Acid Bacteria against Pantoea stewartii Subsp. stewartii of Jackfruit Bronzing Disease. J Food Chem Nanotechnol 9(S1): S627-S634.

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