Background: Cajanus scarabaeoides (family: Fabaceae) is a wild species of the genus Cajanus and it is traditionally used in various healthcare practices. Objective: This study aims to evaluate the seed extracts of C. scarabaeoides for phytochemical content, in-vitro antioxidant activity, and in-silico anti-inflammatory and anti-cancer potential.
Methods: The phytochemical content of C. scarabaeoides seeds was determined by measuring total phenolic contents (TPC), tannin contents (TTC), flavonoid contents (TFC), and the presence of probable compounds established by Gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was analyzed by total antioxidant capacity (TAC), ferric ion reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assays. The molecular docking studies were carried out using AutoDock Vina.
Results: All investigated extracts of C. scarabaeoides showed higher TFC, followed by TTC and TPC (p ≤ 0.05). The antioxidant potential of methanol extract was found significant in TAC, DPPH, and FRAP assays, it showed compelling proof in contrast to the other extracts with a low half-maximal inhibitory concentration (IC50) for TAC (68.83 ± 2.808 μg/ml) and FRAP (53.32 ± 2.82 μg/ml). The GC-MS analysis of methanol extract identified fourteen bioactive compounds and three of them were subjected to molecular docking analysis. The molecular docking results predicted potent anti-inflammatory and anti-cancer potential.
Conclusions: The results have proven the potential antioxidant activity and a possible strong anti-inflammatory and anti-cancer activity for the methanolic seed extracts of C. scarabaeoides. Hence, the isolation and chemical structure identification of bioactive compounds of the methanol seed extract is being carried out.
Citation: Rokkam R, Pinipay F, Bollavarapu A, Rapaka G, Botcha S, et al. 2022. Phytochemical Investigation, Antioxidant Profiling and GC-MS Analysis of Cajanus scarabaeoides Seed Extracts. J Food Chem Nanotechnol 8(4): 147-161.