Biochemical Study on the Anti-Hyperglycemic Effects of Coconut Testa (Cocos nucifera L.) and Red Kidney Bean (Phaseolus vulgaris) Seed Coat in Streptozotocin- Induced Diabetic Rats

Adekola Khatija and Nazrim Marikkar

 

Abstract

Proper management of hyperglycemic condition is the hallmark in the treatment of diabetes mellitus. As plants with good antioxidant potentials are known to have beneficial effect on diabetes and related complications, we aimed at comparing the anti-hyperglycemic activities of the extracts of coconut testa (TOC) and red kidney bean (RKB) seed coat. In this study, the effect of RKB and TOC extracts on Streptozotocin-induced diabetic Sprague-Dawley rats was investigated. Forty-nine male rats were randomly divided into seven groups having seven rats each for normal control, diabetic, and diabetic-treated groups. The oral administration of the two extracts was carried out daily for 14 days at dose levels of 200 and 400 mg/kg b.wt. The anti-hyperglycemic effect of the two extracts were determined using blood glucose levels withdrawn from the animal’s tails after administering glucose at 0, 30, 60 and 120 min. The anti-diabetic effects of the extracts were determined using fasting blood glucose levels after 72 h of injection of extracts. The blood parameters such as cholesterol, urea, bilirubin, creatinine, total protein, Alanine transaminase (ALT), and Aspartate transaminase (AST) were determined using serum isolated from overnight-fasted animals after 14 days of treatment. Results indicated that both TOC and RKB extracts at different doses were capable to reduce hyperglycemia condition significantly (p<0.05). TOC demonstrated the most remarkable reduction at the dose of 200 mg/kg b.wt. Treatment of these two extracts brought significant improvements in some of the blood parameters tested. This study concludes that RKB seed coat and TOC would have high potential to be used as natural sources to prepare food products for diabetic management.

Published on: March 08, 2022
doi: 10.17756/jfcn.2022-120
Citation: Khatija A, Marikkar N. 2022. Biochemical Study on the Anti-Hyperglycemic Effects of Coconut Testa (Cocos nucifera L.) and Red Kidney Bean (Phaseolus vulgaris) Seed Coat in Streptozotocin-Induced Diabetic Rats. J Food Chem Nanotechnol 8(1): 11-17.

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