Spermidine (SPD) is an important signal molecule relate to protein foodsafety, but vulnerable to interference during detection. Here, an electrochemicalsensor system for the spermidine detection was developed by a self-assembledmolecularly imprinted polymer (MIP) film coated on disposable screenprintedelectrodes. The modification imprinted polymer was prepared withtemplate molecules SPD and functional monomer meth acrylic acid (MA) withmolar ratio 1:4 through ultraviolet polymerization. The cyclic and differentialpulse voltammetry (CV and DPV) scanning showed that the best adsorptionequilibrium time of the modified electrodes was 360 s. The electrodes exhibitedgood linear relationship ranging from 1.0 × 10−6 mol/L to 5.0 × 10−6 mol/L (y= −1.22x+7.82, R2 = 0.9952), and the detection limit was 1.7016 × 10−7 mol/L.When take high concentration of histamine and tyramine as interferingcomponents, the modified electrodes detection relative standard deviation was< 5% and thus proved good anti-interference ability. In addition, the densityfunction theory (DFT) calculation explored the intera-molecular interactionof SPD-MA imprinted complex, and the simulation showed that the prepareddevice can keep recognition stability under the potential difference stress rangefrom 0 to 0.005 a.u. Therefore, the prepared system has the potential for rapiddetection spermidine in the place.

PDF (Download Full text)