Auxin is a plant hormone, which mainly includes indole acetic acid (IAA). The study was designed to evaluate and compare different techniques used to check the purity of IAA produced by Phanerochaete chrysosporium. Colorimetric method was used for the qualitative and quantitative determination of crude IAA. Partial purification of IAA was carried out by silica gel column chromatography. UV-Visible spectrophotometric analysis revealed the high similarity of the purified compound with standard IAA. Besides having a comparable UVVisible spectrum, high performance liquid chromatography (HPLC) analysis showed two peaks, which also confirmed the presence of IAA. Thus the article describes efficient production of IAA by Phanerochaete chrysosporium and explores laboratory level experiment design to produce such bioactive compound with different methods to check its purity, which is simple, accurate and rapid to be employed for screening of new compounds.

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