Abstract

This study investigates the phytochemical profile of Tinospora cordifolia methanolic stem extract (TCMSE), a medicinal plant valued in Ayurveda, using gas chromatography-mass spectrometry (GC-MS) analysis to correlate identified compounds with their bioactive properties. TCMSE, known for its immunomodulatory, antioxidant, and anti-inflammatory activities, was analyzed to identify volatile and semi-volatile phytochemicals. The analysis revealed a complex mixture of bioactive compounds, including n-Hexadecanoic acid (Palmitic acid), Benzofuran, and 1,2,3,4-Butanetetrol. Further investigation included antioxidative analysis via 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay of TCMSE, demonstrating significant free radical scavenging activity. Additionally, the anticancer potential of TCMSE was evaluated via 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against K562 and HeLa cancer cell lines, showing promising cytotoxic activity. These findings support the traditional use of TCMSE and pave the way for its application in modern drug development and natural health formulations. The identified phytochemicals offer potential for use in the food, nutraceutical, and pharmaceutical industries, warranting further research into novel drug formulations and dietary products.

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