Abstract
Artificial queen rearing is a revolutionary step for the beekeeping industry where beekeepers quickly multiply the bee population. A successful queen rearing program depends on various factors, including the age of larvae, the priming media, queen cup materials and bee flora. Therefore, this study aimed to evaluate the priming media and queen cup materials (bee wax, paraffin wax, and plastic) on successful queen rearing. The priming media like apple juice, commercial royal jelly enriched with honey, sugar syrup, honey solution, and a mixed solution were treated with 5 μl in each cup materials. The Doolittle grafting method was used to transfer young larvae (less than 24 h old) into queen cups. The result shows that the individual cup materials like bee wax and plastic cup treated with different priming media show significant impact on acceptance of grafted larvae and emergence of virgin queen. The plastic cup was treated with a mixture priming media most increased (90%) acceptance and the virgin queen emergence rate, followed by the bee wax queen cups treated with apple juice (50%). However, there was no impact of priming media treated with paraffin cups on larvae acceptance or queen emergence. Hence, it is concluded that the bee wax and plastic cups used for the artificial queen rearing where individual or mixed priming media performed better in emergence of queen.
doi: 10.17756/jfcn.2025-s1-003
Citation: Alshogari MA, Raut AM, Assouguem A, Gharde SK. 2025. Assessment of Priming Media and Queen Cup Materials in Grafting Techniques for Enhancing Queen Rearing Success. J Food Chem Nanotechnol 11(S1): S15-S20.
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