Bitter melon (Momordica charantia) contains charantin that responsible for the antidiabetic activity. Therefore, it is important to determine charantin in the extracts for the purposed of its formulation or quality control. Herein, we proposed a novel scanometric assay for charantin determination in the bitter melon extracts based on immobilized silver nitrate and methylene blue as a colorimetric paper. The presence of charantin can be detected simply via the color change from blue to green and can be quantified using a scanner via the ImageJ software. The proposed charantin sensor has a response time of 8 mins with the linear range of 300 to 1000 μg/mL and the detection limit was 216.25 μg/mL. The sensor has good reproducibility (RSD = 2.86%) with the stability up-to 5 months. The sensor was applied to determine charantin in the sample extracts, and the result shown in a good agreement with TLC method.
Citation: Afthoni MH, Wahjudi M, Kuswandi B. 2018. Novel Scanometric Assay for Charantin in Bitter Melon (Momordica charantia) Extract Based on Immobilized Silver Nitrate and Methylene Blue as Colorimetric Paper. J Food Chem Nanotechnol 4(2): 27-32.